High-throughput screening of functional deubiquitinating enzymes in autophagy
نویسندگان
چکیده
منابع مشابه
Autophagy-Related Deubiquitinating Enzymes Involved in Health and Disease.
Autophagy is an evolutionarily-conserved process that delivers diverse cytoplasmic components to the lysosomal compartment for either recycling or degradation. This involves the removal of protein aggregates, the turnover of organelles, as well as the elimination of intracellular pathogens. In this situation, when only specific cargoes should be targeted to the lysosome, the potential targets c...
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The promiscuity of a collection of enzymes consisting of 31 wild-type and synthetic variants of CYP1A enzymes was evaluated using a series of 14 steroids and 2 steroid-like chemicals, namely, nootkatone, a terpenoid, and mifepristone, a drug. For each enzyme-substrate couple, the initial steady-state velocity of metabolite formation was determined at a substrate saturating concentration. For th...
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متن کاملA Genomic and Functional Inventory of Deubiquitinating Enzymes
Posttranslational modification of proteins by the small molecule ubiquitin is a key regulatory event, and the enzymes catalyzing these modifications have been the focus of many studies. Deubiquitinating enzymes, which mediate the removal and processing of ubiquitin, may be functionally as important but are less well understood. Here, we present an inventory of the deubiquitinating enzymes encod...
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ژورنال
عنوان ژورنال: Autophagy
سال: 2020
ISSN: 1554-8627,1554-8635
DOI: 10.1080/15548627.2020.1761652